Difference between revisions of "Part:BBa K1587004"
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Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli. | Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli. | ||
<center>[[File:TLSE SHLAGAGA.png]]</center> | <center>[[File:TLSE SHLAGAGA.png]]</center> | ||
− | '''Figure 1: Genetic construct to produce butyrate via glucose''' | + | <center>'''Figure 1: Genetic construct to produce butyrate via glucose'''</center> |
<center>[[File:TLSE ButMetabo.jpg]]</center> | <center>[[File:TLSE ButMetabo.jpg]]</center> | ||
− | '''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli''''' | + | <center>'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli'''''</center> |
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Revision as of 23:24, 17 September 2015
Butyrate synthesis pathway regulated by constitutive promoter p(Bla)
Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 36
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1717
Illegal BamHI site found at 3634
Illegal XhoI site found at 1407 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 332
Illegal AgeI site found at 1254
Illegal AgeI site found at 2900 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1752
Illegal BsaI.rc site found at 2169
Illegal BsaI.rc site found at 3237
Illegal SapI site found at 123
Illegal SapI.rc site found at 3249