Difference between revisions of "Part:BBa K1758343"

Revision as of 23:21, 17 September 2015

MerR activator under constitutive promoter and induceble merT promoter with 5 UTR -sfGFP const. prom + merR+merT-UTR-sfGFP mercury sensor achtivator under the control of a constitutive promoter with mercury induceble promoter and 5´untranslated region which increses the output of the sfGFP which is used for detection.

Usage and Biology

For our sensor, we use parts ( BBa_K346002 and BBa_K346001) of the mercury sensor constructed by iGEM team Peking 2010. These Parts consist of the Mer operon from Shigella flexneri R100 plasmid Tn21, a mercury dependent operon. The expression of the Mer operon is regulated by the activator MerR. The MeR transcription however is regulated by itself. Mercury can bind to the C-terminal located cysteines and generates a conformal change to activate the expression (N.L. Brown et al.2003). Our mercury Sensor contains MerR, which is under control of a constitutive promoter and specific promoter MerT. sfGFP protein is used as measuring output signal and it´s transcription is controlled by the 5` untranslated region, which enhances the following reporter protein sfGFP .

Sequence and Features