Difference between revisions of "Part:BBa K1758334"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1758334 short</partinfo>
 
<partinfo>BBa_K1758334 short</partinfo>
  
−
...
+
Lead induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
<html>
−
<!-- -->
+
This part is essential for our <i> in vitro </i> characterization of our lead sensor.
 +
The edition of T7 promoter to <a href=https://parts.igem.org/Part:BBa_K1758313"target="_blank">BBa_K1758334</a> enables us to characterize tihs sensor in our <a href=http://2015.igem.org/Team:Bielefeld-CeBiTec/Project/CFPS"target="_blank">CFPS</a>.</br>
 +
</html>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K1758334 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1758320 SequenceAndFeatures</partinfo>
−
 
+
−
 
+
−
<!-- Uncomment this to enable Functional Parameter display
+
−
===Functional Parameters===
+
−
<partinfo>BBa_K1758334 parameters</partinfo>
+
−
<!-- -->
+

Revision as of 23:09, 17 September 2015

Lead responsive promoter with T7-promoter and UTR-sfGFP

Lead induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection

Usage and Biology

This part is essential for our in vitro characterization of our lead sensor. The edition of T7 promoter to BBa_K1758334 enables us to characterize tihs sensor in our CFPS.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]