Difference between revisions of "Part:BBa K1615000:Design"
(→Source) |
|||
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | Genomic sequence from Pseudomonas putida. | + | Genomic sequence from <i>Pseudomonas putida</i>. |
===References=== | ===References=== | ||
Revision as of 22:37, 17 September 2015
Morphine-6-dehydrogenase RFC25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 840
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make this gene standardised it was codon optimised for the chassis Esherichia coli as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.
Source
Genomic sequence from Pseudomonas putida.