Difference between revisions of "Part:BBa K1615000:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1615000 short</partinfo> | <partinfo>BBa_K1615000 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | To make this gene standardised it was codon optimised for the chassis <i>Esherichia coli</i> as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence. | |
Revision as of 22:36, 17 September 2015
Morphine-6-dehydrogenase RFC25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 840
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To make this gene standardised it was codon optimised for the chassis Esherichia coli as well as making it RFC25 compatible which required removing all illegal restriction sites in the gene sequence.
Source
Genomic sequence from Pseudomonas putida.