Difference between revisions of "Part:BBa K1585200"
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Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NAD+ dependent alcohol dehydrogenase that converts methanol to formaldehyde.
 
Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NAD+ dependent alcohol dehydrogenase that converts methanol to formaldehyde.
  
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[[File:Aachen_Reaction_mdh.png]]
 
  
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CH<sub>3</sub>OH + NAD(+) → CH<sub>2</sub>O + NADH
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<center>
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<div class="figure" style="float:{{{align|center}}}; margin: 0px 10px 10px 0px; border:{{{border|0px solid #aaa}}};width:{{{width|600px}}};padding:10px 10px 0px 0px;">
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{|
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|<html> <img src="https://static.igem.org/mediawiki/parts/e/e0/Aachen_Reaction_mdh.png" width="600px"></html>
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|-
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|'''{{{title|Catalysed reaction by Mdh}}}'''<br />Methanol + NAD → Formaldehyde + NADH + H{{sup|+}}.
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|}
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</div>
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</center>
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<html></br></html>
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 22:28, 17 September 2015

Mdh2

Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NAD+ dependent alcohol dehydrogenase that converts methanol to formaldehyde.


Catalysed reaction by Mdh
Methanol + NAD → Formaldehyde + NADH + H+.


Usage and Biology

The Characterization was done with RBS B0034 and is documented here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]