Difference between revisions of "Part:BBa K1758325"
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Copper induceble promoter under the control of a T7 with 5´untranslated region infront of a sfGFP wich is used for detection via fluorescence
 
Copper induceble promoter under the control of a T7 with 5´untranslated region infront of a sfGFP wich is used for detection via fluorescence
  
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===Usage and Biology===
 
===Usage and Biology===
 
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The edition of T7 promoter to <a href=https://parts.igem.org/Part:BBa_K1758323"target="_blank">BBa_K1758323</a> enables us to characterize tihs sensor in our <a href=http://2015.igem.org/Team:Bielefeld-CeBiTec/Project/CFPS"target="_blank">CFPS</a>.</br>
 
The edition of T7 promoter to <a href=https://parts.igem.org/Part:BBa_K1758323"target="_blank">BBa_K1758323</a> enables us to characterize tihs sensor in our <a href=http://2015.igem.org/Team:Bielefeld-CeBiTec/Project/CFPS"target="_blank">CFPS</a>.</br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1758325 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1758325 SequenceAndFeatures</partinfo>

Revision as of 22:23, 17 September 2015

Copper responsive promoter with T7-promoter and UTR-sfGFP

Copper induceble promoter under the control of a T7 with 5´untranslated region infront of a sfGFP wich is used for detection via fluorescence


Usage and Biology

This part is essential for our in vitro characterization of our copper sensor. The edition of T7 promoter to BBa_K1758323 enables us to characterize tihs sensor in our CFPS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 29
    Illegal SapI.rc site found at 179