Difference between revisions of "Part:BBa K1692004"
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| − | codon optimized | + | <h2>Introduction</h2> |
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| + | Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli. | ||
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| + | [[File:SB2015_styrene_pathway.png|thumbnail|center|700px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br> | ||
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| + | [[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br> | ||
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| + | <h2>Background</h2> | ||
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| + | [[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br> | ||
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| + | [[File:SB2015_Phe_absorbance.png|thumbnail|center|500px|<b>Phenylalanine Absorbance</b>This is where we write]]<br><br> | ||
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| + | [[File:SB2015_PAL_initial test.png|thumbnail|center|500px|<b>Title</b>This is where we write]]<br><br> | ||
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| + | <h2>Reference</h2> | ||
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Revision as of 22:15, 17 September 2015
codon optimized PAL with T7 promoter and Flag Tag
Introduction
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
Background
File:SB2015 Phe absorbance.png
Phenylalanine AbsorbanceThis is where we write
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1315
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1533
- 1000COMPATIBLE WITH RFC[1000]