Difference between revisions of "Part:BBa K1723005"
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<partinfo>BBa_K1723005 short</partinfo> | <partinfo>BBa_K1723005 short</partinfo> | ||
| − | <b>PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutating a model promoter, PAM rich URS J23117 promoter (BBa_K1723001)</b>. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000), that can only bind in presence of a PAM sequence, as a gene transcription regulator, when in complex with one sgRNA targeting the promoter such as: inhibiting sgRNA X0 (BBa_K1723006), activating sgRNA X4 (BBa_K1723007), or inhibiting sgRNA X35 (BBa_K1723008). | + | <b>PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutating a model promoter [2], PAM rich URS J23117 promoter (BBa_K1723001) [1]</b>. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000), that can only bind in presence of a PAM sequence, as a gene transcription regulator, when in complex with one sgRNA targeting the promoter such as: inhibiting sgRNA X0 (BBa_K1723006), activating sgRNA X4 (BBa_K1723007), or inhibiting sgRNA X35 (BBa_K1723008). |
Discover all the parts that can work with this one: | Discover all the parts that can work with this one: | ||
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<partinfo>BBa_K1723005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1723005 SequenceAndFeatures</partinfo> | ||
| + | ===References=== | ||
| + | |||
| + | [1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437. | ||
| + | |||
| + | [2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763. | ||
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Revision as of 22:07, 17 September 2015
PAM rich URS J23117Alt promoter
PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutating a model promoter [2], PAM rich URS J23117 promoter (BBa_K1723001) [1]. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000), that can only bind in presence of a PAM sequence, as a gene transcription regulator, when in complex with one sgRNA targeting the promoter such as: inhibiting sgRNA X0 (BBa_K1723006), activating sgRNA X4 (BBa_K1723007), or inhibiting sgRNA X35 (BBa_K1723008).
Discover all the parts that can work with this one:
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 277
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 167
Illegal XhoI site found at 195 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.
[2] Alec AK Nielsen & Christopher A Voigt (2014). Multi-input CRISPR/Cas circuits that interface host regulatory network. Molecular systems biology, 10(11), 763.