Difference between revisions of "Part:BBa K1758314"

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<partinfo>BBa_K1758320 short</partinfo></br>
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<partinfo>BBa_K1758320 short</partinfo>
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Chromium induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection
 
Chromium induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection
  

Revision as of 22:01, 17 September 2015

copper activator under control constitutive promoter and strong RBS

Chromium induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection

Usage and Biology

This part is essential for our in vitro characterization of our chromium sensor. The edition of T7 promoter to BBa_K1758313 enables us to characterize tihs sensor in our CFPS.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]