Difference between revisions of "Part:BBa K1692002"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
  
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Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
  
 
[[File:SB2015_styrene_pathway.png|thumbnail|center|700px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br>
 
[[File:SB2015_styrene_pathway.png|thumbnail|center|700px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br>
  
 
[[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br>
 
[[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br>
 +
 +
<h2>Background</h2>
  
 
[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br>
 
[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br>

Revision as of 21:48, 17 September 2015

codon optimized FDC with T7 promoter and Flag Tag

Introduction

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.

Styrene synthesis pathwayThis is where we write


Styrene synthesis enzymesThis is where we write


Background

SDS PAGE Gel of Enzymes for Styrene SynthesisThis is where we write


Styrene synthesis pathwayThis is where we write


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]