Difference between revisions of "Part:BBa K1692002"
Daniel.kunin (Talk | contribs) |
Daniel.kunin (Talk | contribs) |
||
Line 4: | Line 4: | ||
<h2>Introduction</h2> | <h2>Introduction</h2> | ||
+ | Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli. | ||
[[File:SB2015_styrene_pathway.png|thumbnail|center|700px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br> | [[File:SB2015_styrene_pathway.png|thumbnail|center|700px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br> | ||
[[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br> | [[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br> | ||
+ | |||
+ | <h2>Background</h2> | ||
[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br> | [[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br> |
Revision as of 21:48, 17 September 2015
codon optimized FDC with T7 promoter and Flag Tag
Introduction
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
Background
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 233
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]