Difference between revisions of "Part:BBa K1692002"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
  
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[[File:SB2015_styrene_pathway.png|200px|thumb|left|alt text]]
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[[File:SB2015 styrene pathway.png|thumbnail|center|500px|<b>Comparison of P3HB production</b> P3HB extracted from E.coli MG1655 transformed with left to right, control (empty vector), native phaCAB (BBa_K934001) and hybrid promoter phaCAB (BBa_K1149051). Each produced in 300ml cultures of LB with 3% glucose after one night growing at 37 degrees celsius. Imperial iGEM data]]<br><br>
  
  

Revision as of 21:03, 17 September 2015

codon optimized FDC with T7 promoter and Flag Tag

Introduction

Comparison of P3HB production P3HB extracted from E.coli MG1655 transformed with left to right, control (empty vector), native phaCAB (BBa_K934001) and hybrid promoter phaCAB (BBa_K1149051). Each produced in 300ml cultures of LB with 3% glucose after one night growing at 37 degrees celsius. Imperial iGEM data



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]