Difference between revisions of "Part:BBa K1766002"

Latest revision as of 20:27, 17 September 2015

MicF regulated GFP

This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF mRNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silenced in presence of micF.

This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. The BioBrick has been sequenced and confirmed to be the correct construct. When E. coli is transformed with the construct, fluorescent colonies are visible. No further characterisation was attempted due to lack of time.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 861

References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1766002 short</partinfo>
-This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silensed in presence of micF.
+This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non-coding regulatory RNA that binds to the micF target region on the ompF mRNA and prevents it from being translated [1]. Thus, the fusion protein should be consecutively produced under normal conditions and silenced in presence of micF.
-This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. The fluorescent colonies showed the right sequence for the micF target-GFP fusion protein when sequensed using Sanger sequencing. No further characterisation was attempted due to lack of time.
+This part was created as a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015. The BioBrick has been sequenced and confirmed to be the correct construct. When ''E. coli'' is transformed with the construct, fluorescent colonies are visible. No further characterisation was attempted due to lack of time.
 [[File:Fusion protein micf gfp.jpg|200px]]