Difference between revisions of "Part:BBa K1758310"

 
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<partinfo>BBa_K1758310 short</partinfo>
 
<partinfo>BBa_K1758310 short</partinfo>
  
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repressor for chromium responsive promoter ChrP under the control of the konstitutive promoter (K608002)
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Repressor for chromium responsive promoter ChrP under the control of the konstitutive promoter (K608002)
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===Usage and Biology===
 
===Usage and Biology===
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We used this repressor for our chromium sensor. Originaly its from <i>Ochrobactrum triti</i> ci5bvl1. We codon optimized it for use in <i> E.coli</i>. It is essential for our chromium sensor device <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1758313"target="_blank">BBa_K1758313</a>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1758310 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1758310 SequenceAndFeatures</partinfo>

Revision as of 20:25, 17 September 2015

Chromium repressor under control of constitutive promoter and strong RBS

Repressor for chromium responsive promoter ChrP under the control of the konstitutive promoter (K608002)


Usage and Biology

We used this repressor for our chromium sensor. Originaly its from Ochrobactrum triti ci5bvl1. We codon optimized it for use in E.coli. It is essential for our chromium sensor device BBa_K1758313

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 55
    Illegal NheI site found at 966
    Illegal NheI site found at 989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 124
  • 1000
    COMPATIBLE WITH RFC[1000]