Difference between revisions of "Part:BBa K1766002"
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<partinfo>BBa_K1766002 short</partinfo>
 
<partinfo>BBa_K1766002 short</partinfo>
  
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<p>This part consists of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001"> micF target region</a> of the quorum synthase RhlI under control of an OmpR dependent promoter. The promoter is activated by phosphorylated OmpR protein. RhlI produces N-butyryl-homoserine lactone (BHL), a quorum sensing molecule which enables cell to cell signaling.</p>
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This part consists of the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1766001 micF target region] of the ''E. coli'' ompF gene fused to GFP. MicF is a non coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be silensed in presence of micF.  
  
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<p>This part was created to convert the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015, into an extracellular signal that could be detected by a read-out strain.</p>
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This part was created a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.
  
 
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<partinfo>BBa_K1766002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1766002 SequenceAndFeatures</partinfo>
  
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'''References'''
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[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12
  
 
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Revision as of 19:57, 17 September 2015

MicF regulated GFP

This part consists of the micF target region of the E. coli ompF gene fused to GFP. MicF is a non coding regulatory RNA that binds to the micF target region on the ompF RNA and prevents it from being translated [1]. Thus, the fusion protein should be silensed in presence of micF.

This part was created a reporter of the intracellular signal from the chimeric EnvZ-Affibody receptors, created by iGEM Stockholm 2015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861

References [1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12