Difference between revisions of "Part:BBa K1486003:Experience"
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===Applications of BBa_K1486003=== | ===Applications of BBa_K1486003=== | ||
| − | Group: iGEM15_Fudan | + | Group: iGEM15_Fudan |
| − | Author: Xuhang Li | + | Author: Xuhang Li |
Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | ||
Revision as of 19:44, 17 September 2015
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Applications of BBa_K1486003
Group: iGEM15_Fudan Author: Xuhang Li Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR.
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UNIQa98a1fcb2217e33d-partinfo-00000000-QINU UNIQa98a1fcb2217e33d-partinfo-00000001-QINU