Difference between revisions of "Part:BBa K1616008"

 
 
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<partinfo>BBa_K1616008 short</partinfo>
 
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===Usage and Biology===
 
===Usage and Biology===
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This part is the reversed sequence of the HokD; thus this part can be used with a reversed promoter, reversed RBS and reversed terminator.
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The HokD allows an efficient kill-switch.
  
 
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<partinfo>BBa_K1616008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1616008 SequenceAndFeatures</partinfo>
  
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===Design Notes===
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Illegal sites have been checked.
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===Source===
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This part is the reversed sequence of the HokD from BBa_K1497008.
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===References===
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Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.
  
 
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Latest revision as of 18:46, 17 September 2015

HokD reversed

The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane. It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)


Usage and Biology

This part is the reversed sequence of the HokD; thus this part can be used with a reversed promoter, reversed RBS and reversed terminator. The HokD allows an efficient kill-switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal sites have been checked.


Source

This part is the reversed sequence of the HokD from BBa_K1497008.

References

Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.