Difference between revisions of "Part:BBa K1587006"
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<center>[[File:TLSE cI.jpg]]</center>
 
<center>[[File:TLSE cI.jpg]]</center>
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<center>'''Figure 1: Genetic construct'''</center>
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<center>'''Figure 1: Genetic construct for pompC and lacI box regulated cI expression'''</center>
  
 
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Revision as of 15:56, 17 September 2015

pompC and lacI box regulated cI expression

Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly the sequence did not yield any

TLSE cI.jpg
Figure 1: Genetic construct for pompC and lacI box regulated cI expression

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]