Difference between revisions of "Part:BBa K1824018"

 
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<partinfo>BBa_K1824018 short</partinfo>
 
<partinfo>BBa_K1824018 short</partinfo>
  
 
AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.
 
AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.
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===Characterization===
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XJTLU-CHINA tested accumulation status of blue chromoprotein with two different fast digestion tag,lva and aav tag, under the regulation of T7 promter-lac operator in E.coil strain BL21 (DE 3).
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 15:30, 17 September 2015

aeBlue Blue chormoprotein with lva tag

AeBlue chromoprotein could be expressed as a deep blue color in E.coli, which was added by Uppsala University in 2012. We add lva tag to before stop codon to increase the degradation of the Aeblue chromoprotein, which is necessary in our earth map for color changing.

Characterization

XJTLU-CHINA tested accumulation status of blue chromoprotein with two different fast digestion tag,lva and aav tag, under the regulation of T7 promter-lac operator in E.coil strain BL21 (DE 3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]