Difference between revisions of "Part:BBa K1170003:Experience"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 13: Line 12:
 
|width='10%'|
 
|width='10%'|
 
<partinfo>BBa_K1170003 AddReview number</partinfo>
 
<partinfo>BBa_K1170003 AddReview number</partinfo>
−
<I>Username</I>
+
<I>BIT-China</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
 
Enter the review inofrmation here.
 
Enter the review inofrmation here.
 +
 +
== '''Team BIT-China 2015 ''' ==
 +
We mutated the EcoRI site of the promoter by single-step mutation. And we determined the activity of P-atp2 in different pH from 5.0 to 9.0 by measuring the activity of enzyme β-galactosidase(LACZ). As these bacteria grew normally and the OD600 was up to about 1.5, we lysed cells, then added ONPG to start a color reaction and recorded the response time. After the sodium carbonate solution (1M) was added to terminate the reaction, we measured the OD420 and OD550, which could be put into the formula to calculate the activity(Table. 1).
 +
 +
Enzyme Activity= 1000*(OD<sub>420</sub>-1.75*OD<sub>550</sub>)/(t*0.1*OD<sub>600</sub>)
 +
Table. 1 The formula to calculate the enzyme activity of β-galactosidase.
 +
 +
According to the β-galactosidase activities (Fig. 1), we can get the transcription ability of P-atp2 under the different pH environment.
 +
 +
https://static.igem.org/mediawiki/parts/9/9c/BIT_China_parts_P-atp2.png
 +
 +
Fig.1 The β-galactosidase activities in different pH from 5.0 to 9.0.
 +
 +
Team BIT-China 2015 improved this part by making it compatible with RFC10 (see: BBa_K1675022)
 +
 +
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K1170003 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1170003 EndReviews</partinfo>

Revision as of 15:01, 17 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1170003

User Reviews

UNIQ65f5fb8f6d14a27c-partinfo-00000000-QINU UNIQ65f5fb8f6d14a27c-partinfo-00000001-QINU