Difference between revisions of "Part:BBa K1767010"
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<p>The scheme below explains how this BioBrick works :</p> | <p>The scheme below explains how this BioBrick works :</p> | ||
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| + | <p>We performed an experiment to test this part, and here is the result:</p> | ||
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| + | [[File: Technion HS Israel K1767010 result.png|600px|center]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:52, 17 September 2015
P(Lac)IQ RBS LuxR ter ter luxpR RBS eyfp ter ter
We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time.
The here presented BioBrick similar to BBa_k1767009 but without Aiia which consist of a constitutive promoter(I14032) driving expression of LuxR, followed by Lux promoter(R0062) . This promoter is activated by LuxR complex driving expression of YFP.
This BioBrick built for testing because we want to see how the first part of our system works without Aiia .
The scheme below explains how this BioBrick works :
We performed an experiment to test this part, and here is the result:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987