Difference between revisions of "Part:BBa K1587004"

Revision as of 14:37, 17 September 2015

Butyrate synthesis pathway regulated by constitutive promoter p(Bla)

Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly NMR analysis did not yield a significant over production of butyrate in E. coli.

Figure 1: Genetic construct to produce butyrate via glucose

Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 36
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1717
Illegal BamHI site found at 3634
Illegal XhoI site found at 1407
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 332
Illegal AgeI site found at 1254
Illegal AgeI site found at 2900
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1752
Illegal BsaI.rc site found at 2169
Illegal BsaI.rc site found at 3237
Illegal SapI site found at 123
Illegal SapI.rc site found at 3249

@@ Line 6: / Line 6: @@
 <center>[[File:Capture.JPG]]</center>
 '''Figure 1: Genetic construct to produce butyrate via glucose'''
 <center>[[File:TLSE ButMetabo.jpg]]</center>
-'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004'''
+'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli'''''
 <!-- Add more about the biology of this part here