Difference between revisions of "Part:BBa K1696013"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1696013 short</partinfo>
 
<partinfo>BBa_K1696013 short</partinfo>
  
−
The M. florum ssrA tag (mf-ssrA) is degraded by its endogenous Lon protease (mf-Lon) but not by E. coli Lon or ClpXP, and mf-Lon does not recognize or degrade ec-ssrA, providing a protease and cognate degradation tag with orthogonal functionality in E. coli.
+
 
 +
Mf-ssrA tag1 is an identified pdt (protein degradation tag) variant that alters mf-Lon-dependent degradation but not recognition by endogenous ''E.coli'' proteases. In our experiment, we use tag1 as the parental tag and targeted pdt residues 13-15 further for mutagenesis because this region is essential for Lon-mediated degradation in ''Mycoplasma pneumonia'' and does not show homology to known ClpA, ClpX or SspB binding sites.[1]
 +
 
 +
 
 +
== References ==
 +
 
 +
Cameron D E, Collins J J. Tunable protein degradation in bacteria[J]. Nature biotechnology, 2014, 32(12): 1276-1281.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:30, 17 September 2015

Mf-ssrA Tag1


Mf-ssrA tag1 is an identified pdt (protein degradation tag) variant that alters mf-Lon-dependent degradation but not recognition by endogenous E.coli proteases. In our experiment, we use tag1 as the parental tag and targeted pdt residues 13-15 further for mutagenesis because this region is essential for Lon-mediated degradation in Mycoplasma pneumonia and does not show homology to known ClpA, ClpX or SspB binding sites.[1]


References

Cameron D E, Collins J J. Tunable protein degradation in bacteria[J]. Nature biotechnology, 2014, 32(12): 1276-1281.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]