Difference between revisions of "Part:BBa K1864001"
 
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<partinfo>BBa_K1864001 parameters</partinfo>
 
<partinfo>BBa_K1864001 parameters</partinfo>
 
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<h3>IPTG inductive effect</h3>
 
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<p style="margin-right:100px" align="justify">
 
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We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
 
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</p>
 
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<h3>The inductive effect of different concentration IPTG</h3>
 
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We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
 
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[[File:FAFU-CHINA PART (1).png]]
 
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<h3>The inductive effect of different time length</h3>
 
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We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
 
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[[File:FAFU-CHINA PART (2).png]]
 

Latest revision as of 12:50, 17 September 2015

T7 RNA Polymerase

T7 RNA polymerase is used to combine with T7 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1693