We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.
−
</p>
−
−
<br />
−
<h3>The inductive effect of different concentration IPTG</h3>
−
<b>We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours <br />
−
[[File:FAFU-CHINA PART (1).png]]
−
−
<br />
−
<h3>TThe inductive effect of different time length</h3>
−
<b>We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. <br />
−
[[File:FAFU-CHINA PART (2).png]]
Latest revision as of 12:50, 17 September 2015
T7 RNA Polymerase
T7 RNA polymerase is used to combine with T7 promoter.
