Difference between revisions of "Part:BBa K1632013"
Line 6: Line 6:
 
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.
 
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.
  
 +
For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:58, 17 September 2015

PBAD/araC_rbs_fimE(wild-type)

FimE is Fim recombinase.
FimE inverts the fim switch (example BBa_K1632000, BBa_K1632004 etc...) from the ON state to OFF state.
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.

For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1260
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961