Difference between revisions of "Part:BBa K1632006"

Revision as of 09:50, 17 September 2015

fim switch[default ON](Tokyo_Tech/R0010)

The fim switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the fim switch by adding the Fim recombinase.
We designed the fim switch which has a J23119 promoter(BBa_K1632000) . Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech fim switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. This fim switch is an exmple of that. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with wild type fim switch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 539
Illegal BamHI site found at 333
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <span style="margin-left: 10px;">The <i>fim</i> switch is the promoter containing repeated DNA sequence which is inverted by the Fim recombinase. Therefore, we can control the expression of the gene downstream of the <i>fim</i> switch by adding the Fim recombinase.<br>
-<span style="margin-left: 10px;">We designed the ''fim'' switch which has a J23119 promoter(<partinfo>BBa_K1632000</partinfo>) . Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech ''fim'' switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. This ''fim'' switch is an exmple of that. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with Wild Type fim switch.
+<span style="margin-left: 10px;">We designed the ''fim'' switch which has a J23119 promoter(<partinfo>BBa_K1632000</partinfo>) . Also between the promoter and the inverting site, there are two restriction enzyme sites in each front (SalIand BamHI) and back (BglII and MluI)(Fig. 1. Tokyo Tech ''fim'' switch (Tokyo_Tech/J23119) design). So the promoter can easily be interchanged. This ''fim'' switch is an exmple of that. Except for insertion of restriction enzyme sites, basically, the design of TokyoTech fim switch is similar with wild type fim switch.
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