Difference between revisions of "Part:BBa K1686033:Experience"
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To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | To verify <i>OsmY</i> promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase. | ||
− | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. | + | <br>→ As we can see, Curdlan appears after the switch at 27°C. So, <i>OsmY</i> promoter is active in stationary phase only. |
https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png | https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png | ||
<p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> | <p class="reference" align ="center"> <b> Figure 7: <i>OsmY</i> promoter characterization <br> </p> |
Revision as of 09:32, 17 September 2015
Applications of BBa_K1686033
To verify OsmY promoter is only active in the stationary phase, we realized Curdlan quantitative analysis every hour of a culture in LB medium. The switch of temperature for the culture is linked to the transition in stationary phase.
→ As we can see, Curdlan appears after the switch at 27°C. So, OsmY promoter is active in stationary phase only.
Figure 7: OsmY promoter characterization
</p>
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