Difference between revisions of "Part:BBa K1653006"
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The following graphs show that overexpressing marA gives resistance against geraniol and decrease intracelluler geraniol concentration. | The following graphs show that overexpressing marA gives resistance against geraniol and decrease intracelluler geraniol concentration. | ||
<br>'''[https://parts.igem.org/Part:BBa_K1653020 ''marA'' device BBa_K16530020]''' | <br>'''[https://parts.igem.org/Part:BBa_K1653020 ''marA'' device BBa_K16530020]''' | ||
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[[File:GC conc fig.png |650px|thumb|center|Fig. 1: Intracellular geraniol concentrations of ''E. coli'' JM109 (WT) and its overexpressing of ''marA'' strain, ''E. coli'' JM109 (''marA''). | [[File:GC conc fig.png |650px|thumb|center|Fig. 1: Intracellular geraniol concentrations of ''E. coli'' JM109 (WT) and its overexpressing of ''marA'' strain, ''E. coli'' JM109 (''marA''). | ||
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Revision as of 09:27, 17 September 2015
MarA
The activator of AcrAB-TolC multidrug efflux pump exports some terpenes
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA device as BBa_K1653006 to iGEM Registry.
In exsisting part's information of marA, it gives E. coli resistance against kanamycin only.
In this year, we confilmed that overepressing of marA gives E. coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of toxic organic substances that are produced using bacteria.
[http://2015.igem.org/Team:Nagahama/Medal_Parts#Gold Here] is our result page.
Export geraniol
The following graphs show that overexpressing marA gives resistance against geraniol and decrease intracelluler geraniol concentration.
marA device BBa_K16530020
Resistance
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000COMPATIBLE WITH RFC[1000]