Difference between revisions of "Part:BBa K1230000:Experience"
(→Applications of BBa_K1230000) |
(→User Reviews) |
||
Line 32: | Line 32: | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1230000 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1230000 StartReviews</partinfo> | ||
− | + | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
|- | |- |
Revision as of 08:15, 17 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1230000
We improved the characterization of a previously existing BioBrick Part BBa_K1230000 and submitted this improved BioBrick marA device as BBa_K1653006 to iGEM Registry.
In exsisting part's information of marA, it gives E. coli resistance against kanamycin only. In this year, we confilmed that overepressing of marA gives E. coli resistance against geraniol as one of the terpene and decrease its intracellular concentration. This information is very beneficial for other iGEMers to production of organic substance that have toxicity using bacteria.
The activator of AcrAB-TolC multidrug efflux pump exports some terpenes
![](/wiki/images/c/c1/GC_conc_fig.png)
In this figure, intracellular content of geraniol was less in the strain E. coli JM109 (marA) than the strain E. coli JM109 (WT). The concentrations of intracellular geraniol from E. coli JM109 (marA) was 42.9 μg/ml, which was 40% lower than that from of E. coli JM109 (WT), 72.2 μg/ml. This figure is suggesting that internalized geraniol could be more efficiently exported through AcrAB-TolC efflux pump following the presumed activation of this gene by introducing the activator marA gene.
Resistance
![](/wiki/images/4/41/MarA_plate_assay_yoshiharu_did.png)
![](/wiki/images/4/4d/MarA_plate_assay_nishikawa_last.png)
User Reviews
UNIQb6850228cc7acb36-partinfo-00000000-QINU
No review score entered. Username |
We were able to characterise this part, since we found a measurable phenotype (kanamycin resistance).
A test was performed with three different antibiotics and different IPTG concentrations. The part seems to make the host sensible to Tetracyclin (maybe because it's on Cm plasmid, and both antibiotics act on similar ways), it seems not to have an effect on Ampicilin and it shows a relevant effect with Kanamycin. Which is further explored in the next figure.
|
UNIQb6850228cc7acb36-partinfo-00000002-QINU