Difference between revisions of "Part:BBa K1755001"

Latest revision as of 07:59, 17 September 2015

ribB coding sequence

ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase;

We get this part from E.coli genome by synthetize. It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage in MFC

Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low. Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.

Usage and Biology

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.

This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.

Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Sequence (amino acid)

       10         20         30         40         50         60         70         80         90        100

TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM

       110        120       130        140        150        160        170        180        190        200

GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR

TIEDLVAYRQ AHERKAS

Figure 1:Normal E.coli(left one)and E.coli with ribB(right one) Figure 2:Constitutive parts riboflavin expression level

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 387
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 277
Illegal AgeI site found at 319
1000
COMPATIBLE WITH RFC[1000]

references

1.UniPort P0A7J0 RIBB_ECOLI [http://www.uniprot.org/uniprot/P0A7J0]

2.iGEM 2014 LZU-China Part:BBa_K1523101 [1]

3.Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.Richter G., Volk R., Krieger C., Laham H.-W., Roethlisberger U., Bacher A.J. Bacteriol. 174:4050-4056(1991）

@@ Line 1: / Line 1: @@
 <partinfo>BBa_K1755001 short</partinfo>
-ribB&#65292;the 3,4 dihydroxy-2-butanone-4-phosphate synthase&#12290;
+ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase;
 We get this part from E.coli genome by synthetize.
 It can increase the electric output by synthesis of riboflavin.
@@ Line 9: / Line 10: @@
 == Usage in MFC ==
-[[File:IGEM_2015_MFC.JPG|300px]]
+[[File:IGEM_2015_MFC.JPG|600px]]
 Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.