Difference between revisions of "Part:BBa K1755001"
 
(28 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
[[File:Example.jpg]]__NOTOC__
 
 
<partinfo>BBa_K1755001 short</partinfo>
 
<partinfo>BBa_K1755001 short</partinfo>
  
−
ribB&#65292;the 3,4 dihydroxy-2-butanone-4-phosphate synthase&#12290;
+
ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase;
−
We get this part from E.coli genome by PCR.
+
 
 +
We get this part from E.coli genome by synthetize.
 
It can increase the electric output by synthesis of riboflavin.
 
It can increase the electric output by synthesis of riboflavin.
  
Line 9: Line 9:
 
After the addition of ribB, the bacteria will be yellow.
 
After the addition of ribB, the bacteria will be yellow.
  
−
===Usage and Biology===
+
== Usage in MFC ==
 +
[[File:IGEM_2015_MFC.JPG|600px]]
 +
 
 +
Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.
 +
Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.
 +
 
 +
== Usage and Biology ==
  
 
The protein RibB catalyzes the conversion of  
 
The protein RibB catalyzes the conversion of  
Line 27: Line 33:
 
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
 
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
  
−
== Sequence ==
 
  
−
         10        20        30        40        50
+
== Sequence (amino acid) ==
−
MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM
+
         10        20        30        40        50        60        70        80        90        100
−
         60        70        80        90        100
+
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM
−
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE  
+
        110        120       130        140        150        160        170        180        190        200
−
      110        120       130        140        150
+
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR
−
AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR
+
        210  
−
       160        170        180        190        200
+
TIEDLVAYRQ AHERKAS
−
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV  
+
−
      210  
+
−
TIEDLVAYRQ AHERKAS  
+
  
−
[[File:LZU_iGEM_2015_ribB_picture.JPG|400px|thumb|left|Normal E.coli(left one)and E.coli with ribB(right one)]]
+
----
 +
 
 +
[[File:LZU_iGEM_2015_ribB_picture.JPG|400px]]
 +
Figure 1:Normal E.coli(left one)and E.coli with ribB(right one)
 +
[[File:Constitutive.png]]
 +
Figure 2:Constitutive parts riboflavin expression level
  
 
<!-- -->
 
<!-- -->
Line 51: Line 58:
 
<partinfo>BBa_K1755001 parameters</partinfo>
 
<partinfo>BBa_K1755001 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
== references ==
 +
1.UniPort  P0A7J0  RIBB_ECOLI [http://www.uniprot.org/uniprot/P0A7J0]
 +
 +
2.iGEM 2014 LZU-China Part:BBa_K1523101 [https://parts.igem.org/Part:BBa_K1523101]
 +
 +
3.Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.Richter G., Volk R., Krieger C., Laham H.-W., Roethlisberger U., Bacher A.J. Bacteriol. 174:4050-4056(1991）

Latest revision as of 07:59, 17 September 2015

ribB coding sequence

ribB;the 3,4 dihydroxy-2-butanone-4-phosphate synthase;

We get this part from E.coli genome by synthetize. It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage in MFC

IGEM 2015 MFC.JPG

Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low. Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.

Usage and Biology

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.

This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.

Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.


Sequence (amino acid)

       10         20         30         40         50         60         70         80         90        100

TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE MNQTLLSSFG TPFERVENAL AALREGRGVM VLDDEDRENE GDMIFPAETM 

       110        120       130        140        150        160        170        180        190        200

GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIE FANKHNMALV AAEGVTTGVS AADRITTVRA AIADGAKPSD LNRPGHVFPL RAQAGGVLTR 

       210

TIEDLVAYRQ AHERKAS

LZU iGEM 2015 ribB picture.JPG Figure 1:Normal E.coli(left one)and E.coli with ribB(right one) Constitutive.png Figure 2:Constitutive parts riboflavin expression level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


references

1.UniPort P0A7J0 RIBB_ECOLI [http://www.uniprot.org/uniprot/P0A7J0]

2.iGEM 2014 LZU-China Part:BBa_K1523101 [1]

3.Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.Richter G., Volk R., Krieger C., Laham H.-W., Roethlisberger U., Bacher A.J. Bacteriol. 174:4050-4056(1991）