Difference between revisions of "Part:BBa K1632013"

Revision as of 05:47, 17 September 2015

PBAD/araC_rbs_fimE(wild-type)

FimE is Fim recombinase.
FimE inverts the fim switch (example BBa_K1632000, BBa_K1632004 etc...) from the ON state to OFF state.
The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 1260
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 3: / Line 3: @@
 FimE is Fim recombinase.<br>
-With the expression of FimE, the <i>fim</i> switch (example <partinfo>BBa_K1632000</partinfo>, <partinfo>BBa_K1632004</partinfo> etc...) only inverts from the ON state to OFF state.<br>
+FimE inverts the <i>fim</i> switch (example <partinfo>BBa_K1632000</partinfo>, <partinfo>BBa_K1632004</partinfo> etc...) from the ON state to OFF state.<br>
 The expression of this Fim recombinase is controlled by arabinose in BBa_K1632013.