Difference between revisions of "Part:BBa K1592008:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.
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In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.Also we added 6Xhis-tag between  LIP2 prepro and E. coli ribosomal protein L2 to do verification of immunofluorescence.
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===Source===
 
===Source===

Revision as of 04:46, 17 September 2015


LIP prepro + E. coli ribosomal protein L2 (61-202aa) + YLcwp3 Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 641


Design Notes

In order to replace the domains more conveniently, we added BamHI, SalI, NdeI, NdeI between LIP2 prepro, E. coli ribosomal protein L2, GS linker and YLcwp3.Also we added 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 to do verification of immunofluorescence.

Source

E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.

References