Difference between revisions of "Part:BBa K1820014"

Revision as of 04:34, 17 September 2015

CP8_RBS_sfGFP(Bs)_Terminator

Usage and Biology

This is a composite part designed as a report protein sequence for use in Lactococcus lactis. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for Lactococcus lactis followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for Bacillus subtilis (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for L. lactis, it has displayed function in Escherichia coli. The sfGFP(Bs) has worked well in a variety of bacteria, such as L. lactis, B. subtilis, Escherischia coli and Streptococcus pneumoniae and there are indications that the promoter is functional in a large number of prokaryotic organisms, so it is likely that this construct will be functional in a variety of prokaryotic organisms.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 99

References

Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/

Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79: 6481-6490

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 <p> This is a composite part designed as a report protein sequence for use in ''Lactococcus lactis''. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for ''Lactococcus lactis'' followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for ''Bacillus subtilis'' (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for ''L. lactis'', it has displayed function in ''Escherichia coli''. The sfGFP(Bs) has worked well in a variety of bacteria, such as ''L. lactis'', ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae'' and there are indications that the promoter is functional in a large number of prokaryotic organisms, so it is likely that this construct will be functional in a variety of prokaryotic organisms.</p>
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
+<span class='h3bb'></span>
 <partinfo>BBa_K1820014 SequenceAndFeatures</partinfo>
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 <partinfo>BBa_K1820014 parameters</partinfo>
 <!-- -->
 ===References===
 <p>Jensen, P. R., Hammer, K. (1998). The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters. Appl Environ Microbiol. 1998 Jan; 64(1): 82–87. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/</p>
 <p>Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in ''Bacillus subtilis'', ''Streptococcus pneumoniae'', and ''Lactococcus lactis'' for live cell imaging. ''Appl. Environ. Microbiol.'' 79: 6481-6490</p>