Difference between revisions of "Part:BBa K1820014"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | <p> This is a composite part designed as a report protein sequence for use in ''Lactococcus lactis''. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for ''Lactococcus lactis'' followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for ''Bacillus subtilis'' (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for ''L. lactis'', it has displayed function in ''Escherichia coli''. The sfGFP(Bs) has worked well in a variety of bacteria, such as ''L. lactis', ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae'' and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.</p> | + | <p> This is a composite part designed as a report protein sequence for use in ''Lactococcus lactis''. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for ''Lactococcus lactis'' followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for ''Bacillus subtilis'' (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for ''L. lactis'', it has displayed function in ''Escherichia coli''. The sfGFP(Bs) has worked well in a variety of bacteria, such as ''L. lactis'', ''B. subtilis'', ''Escherischia coli'' and ''Streptococcus pneumoniae'' and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.</p> |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 04:25, 17 September 2015
CP8_RBS_sfGFP(Bs)_Terminator
Usage and Biology
This is a composite part designed as a report protein sequence for use in Lactococcus lactis. The promoter is a mid-range promoter from Peter Ruhdal Jensen and Karin Hammer's library of synthetic promoters for Lactococcus lactis followed by a popular ribosome binding site (Elowitz 1999), a super folded green fluorescent protein codon optimized for Bacillus subtilis (sfGFP(Bs)), and a popular double-stop terminator. Although it was designed for L. lactis, it has displayed function in Escherichia coli. The sfGFP(Bs) has worked well in a variety of bacteria, such as L. lactis, B. subtilis, Escherischia coli and Streptococcus pneumoniae and there are indications that the promoter is functional in a large number of prokaryotic organisms as well, so it is likely that this construct will be functional in a variety of prokaryotic organisms.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 99