Difference between revisions of "Part:BBa K299812:Experience"

Revision as of 03:53, 17 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K299812

User Reviews

UNIQ824588371b9f6203-partinfo-00000000-QINU

No review score entered. yunting from SPSingapore 2015

The sequence of the part was significantly different as was documented. Our team had trouble sequencing it initially as the full suffix sequence was absent, and primers that were designed based on the documented nucleotide sequence didn't work. We had to use universal primers to sequence the first 1kb, then design new primers for the next kb. Thus we had 3 staggered rounds of sequencing.

UNIQ824588371b9f6203-partinfo-00000002-QINU

UNIQ824588371b9f6203-partinfo-00000003-QINU

@@ Line 11: / Line 11: @@
 |width='10%'|
 <partinfo>BBa_K299812 AddReview number</partinfo>
-<I>yunting</I> from SPSingapore 2015
+<I>'''yunting''' from SPSingapore 2015</I>
 |width='60%' valign='top'|
-The sequence of the part was significantly different as documented.
+The sequence of the part was significantly different as was documented. Our team had trouble sequencing it initially as the full suffix sequence was absent, and primers that were designed based on the documented nucleotide sequence didn't work. We had to use universal primers to sequence the first 1kb, then design new primers for the next kb. Thus we had 3 staggered rounds of sequencing.
 |}
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K299812 EndReviews</partinfo>
 <!-- Template for a user review