Difference between revisions of "Part:BBa K1819008"

Revision as of 02:14, 17 September 2015

Promoter test circuit to analyze the kill switch efficiency

BBa_K1819008 construction indicates lactose promoter (pLac) efficiency to control a TetR promoter inhibited by TetR protein. This circuit was used to verify a kill switch mechanism (See more details in [http://2015.igem.org/Team:Brasil-USP]<a href=http://2015.igem.org/Team:Brasil-USP>Brasil-USP</a> team wiki)

In the absence of pLac inductor (Lactose or IPTG), RFP will be constitutively expressed under TetR promoter control. If inducer is added, TetR promoter is repressed and, consequently, RFP expression ceases and GFP expression starts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2437
Illegal AgeI site found at 2549
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 870

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1819008 short</partinfo>
-BBa_K1819008 construction indicates lactose promoter (pLac) efficiency to control a TetR promoter inhibited by TetR protein. This circuit was used to verify a kill switch mechanism (See more details in <a href=http://2015.igem.org/Team:Brasil-USP>Brasil-USP</a> team wiki)
+BBa_K1819008 construction indicates lactose promoter (pLac) efficiency to control a TetR promoter inhibited by TetR protein. This circuit was used to verify a kill switch mechanism (See more details in [http://2015.igem.org/Team:Brasil-USP]<a href=http://2015.igem.org/Team:Brasil-USP>Brasil-USP</a> team wiki)
 In the absence of pLac inductor (Lactose or IPTG), RFP will be constitutively expressed under TetR promoter control. If inducer is added, TetR promoter is repressed and, consequently, RFP expression ceases and GFP expression starts.