Difference between revisions of "Part:BBa K1871001"

Revision as of 00:05, 17 September 2015

Alpha amylase with native secretion

This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence (BBa_K165002) the ADH1 terminator (BBa_K392003). Expression is controlled by the minimal cyc yeast promoter (Part BBa_K105027).

Minimal cyc promoter

This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.

Kozak Sequence

This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

Native Alpha Amylase Secretion Sequence A signal sequence which directs the protein to be secreted.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 105
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1533
Illegal NgoMIV site found at 1548
Illegal AgeI site found at 1851
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence (BBa_K165002) the ADH1 terminator (BBa_K392003). Expression is controlled by the minimal cyc yeast promoter (Part BBa_K105027).
+====Minimal cyc promoter====
+This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.
 ====Kozak Sequence====
 This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.
+Native Alpha Amylase Secretion Sequence
+A signal sequence which directs the protein to be secreted.
 ====Alpha Amylase====
@@ Line 15: / Line 21: @@
 This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.
-====Minimal cyc promoter====
-This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.
 <!-- Add more about the biology of this part here