Difference between revisions of "Part:BBa K1678001"

 
 
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<partinfo>BBa_K1678001 short</partinfo>
 
<partinfo>BBa_K1678001 short</partinfo>
  
 
Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase.
 
Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase.
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Coding sequence has been codon optimized for Lactobacillus plantarum. The gene is promoted by the synthetic promoter p25 from 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.
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Coding sequence has been codon optimized for Lactobacillus plantarum.
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Protein is expressed by the L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019).
 
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141)
 
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141)
 
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
 
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).
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The expressed protein catalyses two steps in the riboflavin pathway.
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The expressed protein catalyses one step in the riboflavin pathway.
  
  

Latest revision as of 23:23, 16 September 2015

Pyrimidine reductase/pyrimidine deaminase cassette RibD

Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. Coding sequence has been codon optimized for Lactobacillus plantarum. Protein is expressed by the L.plantarum synthetic promoter p25 (A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum, Microbiology (2006), 152, 1011–1019). The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses one step in the riboflavin pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1262