Difference between revisions of "Part:BBa K1678001:Design"

 
 
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<partinfo>BBa_K1678001 short</partinfo>
 
<partinfo>BBa_K1678001 short</partinfo>

Latest revision as of 22:32, 16 September 2015

Pyrimidine reductase/pyrimidine deaminase cassette RibD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1262


Design Notes

RibD is not considered as a bottleneck in the riboflavin pathway. A medium promoter is so expressing it.



Source

Coding sequence comes from Bacillus subtilis genome sequence. It has been codon optimized for Lactobaciilus plantarum on IDT website. p25 is a synthetic constitutive promoter designed in 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006. The RBS informations are coming from Tauer et al. Microbial Cell Factories 2014, 13:150. Tldh terminator information are documented in Spath et al. Microbial Cell Factories 2012, 11:141.


References