Difference between revisions of "Part:BBa K1678001"
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<partinfo>BBa_K1678001 short</partinfo> | <partinfo>BBa_K1678001 short</partinfo> | ||
Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. | Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. | ||
− | Coding sequence has been codon optimized for Lactobacillus plantarum. | + | Coding sequence has been codon optimized for Lactobacillus plantarum. |
+ | Protein is expressed by the strong L.plantarum synthetic promoter BBa_K1678004. | ||
The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) | The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) | ||
The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). | The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). |
Revision as of 22:32, 16 September 2015
Pyrimidine reductase/pyrimidine deaminase cassette RibD
Bacillus subtilis RibD gene for riboflavin production. RibD code for the Pyrimidine reductase/deaminase. Coding sequence has been codon optimized for Lactobacillus plantarum. Protein is expressed by the strong L.plantarum synthetic promoter BBa_K1678004. The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141) The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150). The expressed protein catalyses two steps in the riboflavin pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1262