Difference between revisions of "Part:BBa K1723005"

Revision as of 21:50, 16 September 2015

PAM rich URS J23117Alt promoter

<b/>PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutation of PAM rich URS J23117 promoter (BBa_K1723001)</b>. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008). dCas9 can only bind in presence of a PAM sequence.

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http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 277
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 167
Illegal XhoI site found at 195
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1723005 short</partinfo>
-PAM rich URS J23117 is a promoter made from the existing PAM rich URS J23117 promoter (BBa_K1723001) and mutated. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008).
+<b/>PAM rich URS J23117Alt is a new fully synthetic promoter obtained by mutation of PAM rich URS J23117 promoter (BBa_K1723001)</b>. It acts the exactly same way as the PAM rich URS J23117 promoter but is targeted by others specific sgRNAS. See the design section for more information about its creation. It has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008). dCas9 can only bind in presence of a PAM sequence.
 Discover all the parts that can work with this one: