Difference between revisions of "Part:BBa K1725083"

 
 
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<partinfo>BBa_K1725083 short</partinfo>
 
<partinfo>BBa_K1725083 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1725083 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1725083 SequenceAndFeatures</partinfo>
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This construct was used as a control to characterise repression of K1725000 by K1725040.
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R0040 driven expression is repressed by C0040 (TetR repressor) as shown in the figure below. K1725082 is GFP expression driven by R0040 (TetR repressible promoter).
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https://static.igem.org/mediawiki/2015/9/95/Glasgow_2015_Repression_Fold_Graph.png
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<b>Represser constructs with pSB1C3 backbone; promoter driving GFP constructs with pSB3K3 backbone. Repressor protein expression induced with 100μM IPTG. Replicates of constructs and controls of three dilutions from one colony, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.</b>
  
  

Latest revision as of 20:38, 16 September 2015

R0011.B0032.C0040

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This construct was used as a control to characterise repression of K1725000 by K1725040.

R0040 driven expression is repressed by C0040 (TetR repressor) as shown in the figure below. K1725082 is GFP expression driven by R0040 (TetR repressible promoter).


Glasgow_2015_Repression_Fold_Graph.png

Represser constructs with pSB1C3 backbone; promoter driving GFP constructs with pSB3K3 backbone. Repressor protein expression induced with 100μM IPTG. Replicates of constructs and controls of three dilutions from one colony, under the same conditions. Mean and standard deviation of replicates were calculated to give value and error bars.