Difference between revisions of "Part:BBa K1725020:Design"

 
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===Design Notes===
 
===Design Notes===
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We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html They based the promoter sequence on J23119, then overlapped the operator sequence of the SrpR repressor over the -10 site.
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We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html
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Stanton et al., (2014) overlapped the operator sequence for the SrpR repressor over the -10 and/or -35 sites of BBa_J23119, a strong, constitutive Anderson family promoter, meaning when the repressor binds to its operator sequence sigma factor cannot recognise the promoter (and therefore attract RNA polymerase) and transcription cannot start.
  
  

Revision as of 20:19, 16 September 2015

SrpR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the promotor sequence from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

Stanton et al., (2014) overlapped the operator sequence for the SrpR repressor over the -10 and/or -35 sites of BBa_J23119, a strong, constitutive Anderson family promoter, meaning when the repressor binds to its operator sequence sigma factor cannot recognise the promoter (and therefore attract RNA polymerase) and transcription cannot start.


Source

From "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

References