Difference between revisions of "Part:BBa K1679010:Design"

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===Design Notes===
 
===Design Notes===
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We use a special RBS to make the device temperature-sensitive.When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded.Thus the ribosome can't translated RFP.And when the temperature rises above 42°C,the secondary structure of the RBS after transform would be unfolded and produce RFP.However,according to our experience,it didn't appear red when the temperature rises above 42°C.
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We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.
  
 
===Source===
 
===Source===

Revision as of 20:08, 16 September 2015

promoter+RNA thermometer+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 700
    Illegal AgeI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.

Source

BBa_J23106

BBa_K115002

BBa_E1010

BBa_B0015

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.