Difference between revisions of "Part:BBa K1723001"

Revision as of 19:45, 16 September 2015

PAM rich URS j23117 promoter

PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z0 (BBa_K1723002), the inhibitor sgRNA Z35 (BBa_K1723003), or the other inhibitor sgRNA Z4 (BBa_K1723004). dCas9 can only bind if its target sequence is preceded PAM sequence. Discover the applications of this part in the tab 'experience'.

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http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 56
Illegal NheI site found at 79
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 43
1000
COMPATIBLE WITH RFC[1000]

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−	<b>PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter</b>. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z0 (BBa_K1723002), the inhibitor sgRNA Z35 (BBa_K1723003), or the other inhibitor sgRNA Z4 (BBa_K1723004). dCas9 can only bind if its target sequence is preceded PAM sequence. Discover the applications of this part in the tab 'experience'~~. This protein is only active in the JEN202 ''E. coli'' strain [1]~~ .	+	<b>PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter</b>. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z0 (BBa_K1723002), the inhibitor sgRNA Z35 (BBa_K1723003), or the other inhibitor sgRNA Z4 (BBa_K1723004). dCas9 can only bind if its target sequence is preceded PAM sequence. Discover the applications of this part in the tab 'experience'.

	Discover all the parts that can work with this one:		Discover all the parts that can work with this one: