Difference between revisions of "Part:BBa K1660001"
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| + | == rMpL is a typical kind of β-trefoil folded lectin, consisting of α-,β-;s and γ- repeats. The sequence of gene rMpL is 582bp long,we build this part by linking a pBAD promoter(BBa_K206000) and RBS(BBa_B0034) in the upstream region, of course we did | ||
| + | restriction endonuclease and SDS-PAGE to festifytg thep expression of rMpl. | ||
| + | |||
| + | Whats'more,we also desiganed toxicity test for nematodes,the results will be showed in the | ||
== Headline text == | == Headline text == | ||
== Headline text == | == Headline text == | ||
Revision as of 18:55, 16 September 2015
rMpL
This part encodes a nematotoxic lectin from the parasol mushroom( Macrolepiota procera) under the control of pBAD promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 151 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 208
Illegal AgeI site found at 304
Illegal AgeI site found at 519 - 1000COMPATIBLE WITH RFC[1000]
Media:BNU-MODELING-DEVICE-VIDEO.mp4
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== rMpL is a typical kind of β-trefoil folded lectin, consisting of α-,β-;s and γ- repeats. The sequence of gene rMpL is 582bp long,we build this part by linking a pBAD promoter(BBa_K206000) and RBS(BBa_B0034) in the upstream region, of course we did
restriction endonuclease and SDS-PAGE to festifytg thep expression of rMpl.
Whats'more,we also desiganed toxicity test for nematodes,the results will be showed in the