Difference between revisions of "Part:BBa K1604019"
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We placed BBa_K1604019 under a costituve promoter and we characterized blh. <br> | We placed BBa_K1604019 under a costituve promoter and we characterized blh. <br> | ||
To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604021#Usage_and_Biology">BBa_K1604021</a></html> and the part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1604022">BBa_K1604022</a></html> | To see the characterization results check the part <html><a href="https://parts.igem.org/Part:BBa_K1604021#Usage_and_Biology">BBa_K1604021</a></html> and the part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1604022">BBa_K1604022</a></html> | ||
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Check out our Wiki <html><a href="http://2015.igem.org/Team:UNITN-Trento">UNITN-Trento iGEM 2015</a>! </html> | Check out our Wiki <html><a href="http://2015.igem.org/Team:UNITN-Trento">UNITN-Trento iGEM 2015</a>! </html> | ||
Revision as of 16:53, 16 September 2015
RBS + β-carotene 15,15'-dioxygenase (blh gene)
This part contains RBS and blh gene. β-carotene 15,15’-dioxygenase is involved in retinal biosynthesis and catalizes the last step of the pathway by cleaving one molecule of β-carotene into two molecules of retinal. Blh is the gene that encodes for this enzyme, and was first isolated in the uncultured bacteria SAR86 [1] . Blh is present in other organisms (i.e. cyanobacteria and algae) however enzymes involved in the cleavage of β-carotene that involve different mechanisms are found in many other organisms.
The enzyme requires oxygen for the cleavage of β-carotene, which was demonstrated to be provided by water [2] . This data are consistent with the fact the bacteria lives in deep marine waters. It requires Fe2+ that is coordinated by 4 His residues that are highly conserved.
Usage and Biology
We placed BBa_K1604019 under a costituve promoter and we characterized blh.
To see the characterization results check the part BBa_K1604021 and the part BBa_K1604022
Check out our Wiki UNITN-Trento iGEM 2015!
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Martinez A.,"Proteorhodopsin Photosystem Gene Expression Enables Photophosphorylation in a Heterologous Host" Proceedings of the National Academy of Sciences 104.13 (2007): 5590-595. Web
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Yeong-Su Kim, Retinal production from b-carotene by b-carotene 15,150-dioxygenase from an uncµlturable marine bacterium, Biotechnol Lett (2010) 32:957–961
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 202
- 1000COMPATIBLE WITH RFC[1000]
Sequence and Features