Difference between revisions of "Part:BBa K1767009"
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| + | <p>We performed an experiment to test this part, and here is the result:</p> | ||
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Revision as of 16:50, 16 September 2015
P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS eyfp ter ter
We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time.
In order to understand our system we started to test the first part of our genetic circuit , to test it we built the first part ( k1767001 ) driving expression of florescence protein .
The here presented BioBrick consists of a constitutive promoter(I14032) driving expression of LuxR followed by a Lux promoter (R0062) , driving expression of YFP.
The idea is to retrieve information about the expression of a fluorescent reporter downstream of the pLuxR promoter in absence and increasing concentrations of AHL.
The scheme below explains how this BioBrick works :
We performed an experiment to test this part, and here is the result:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 157
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1761